NASC Stock Detail Page
NASC ID: N2107774
Name: H2B::GFP
ABRC stock number: CS2107774
Description: cDNAs used for the generation of most of these constructs were available as trimmed U clones (cDNAs trimmed to the annotated start and stop codons in the Cre-Lox pUNI vector) from the SSP Orfeome collection (http://signal.salk.edu/SSP/). In other cases the cDNA was generated by PCR from an Arabidopsis thaliana cDNA library.Coding regions with stop-codon deleted were amplified by high-fidelity PCR and then inserted by recombinase cloning into a standard Gateway entry vector (pDONR207; www.invitrogen.com). Entry vector clones were transferred by recombinase cloning, into Gateway binary plant expression vectors GFP-N-BIN and pROK2-mRFP (which contains the coding sequence of monomeric RFP (Campbell et al., 2002) at the C-terminal end of the AttR cassette). Agrobacterium mediated transformation was used to create GFP-NBIN and mRFP construct transformed lines.
Donation Date: 2018-03-23
| Donated by: | John Innes Centre Peter Shaw |
Part of : The Plant Nuclear Marker Collection
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Seed type: Transgenic
Mutagen: T-DNA Insertion
Background: col-0
Growth requirement: none
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
No visible phenotype
References
Rosa, S. et al; 2014-01-01; Cell differentiation and development in Arabidopsis are associated with changes in histone dynamics at the single-cell level ; The Plant Cell; 26; 12; 4821–4833; PubMed ID: 25549670